Designed by: Yiran Wu Group: iGEM14_SCUT-China (2014-10-07)
pT7-RBS+loading module+KS-AT1+KR1+ACP+KS-AT2
Assembly Compatibility:
10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1880 Illegal BamHI site found at 6421
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 558 Illegal NgoMIV site found at 2043 Illegal NgoMIV site found at 2415 Illegal NgoMIV site found at 2448 Illegal NgoMIV site found at 2921 Illegal NgoMIV site found at 3630 Illegal NgoMIV site found at 4501 Illegal AgeI site found at 369 Illegal AgeI site found at 1733 Illegal AgeI site found at 2079 Illegal AgeI site found at 2658 Illegal AgeI site found at 4377 Illegal AgeI site found at 4447 Illegal AgeI site found at 5074
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1483 Illegal BsaI.rc site found at 3915 Illegal SapI.rc site found at 2750
Design Notes
Source
Saccharopolyspora erythraea
References
[1]Cane, David E. Programming of erythromycin biosynthesis by a modular polyketide synthase [J]. Journal of Biological Chemistry, 2010, 285.36: 27517-27523.
[2]Komaki, Hisayuki, et al. Genome based analysis of type-I polyketide synthase and nonribosomal peptide synthetase gene clusters in seven strains of five representative Nocardia species [J]. BMC genomics 15.1 (2014): 323.
[3]Pfeifer, Blaine A., et al. Biosynthesis of complex polyketides in a metabolically engineered strain of E. coli [J]. Science, 2001, 291.5509: 1790-1792.
[4]Tae, Hongseok, Jae Kyung Sohng, and Kiejung Park. Development of an analysis program of type I polyketide synthase gene clusters using homology search and profile hidden Markov model [J]. Journal of microbiology and biotechnology, 2009, 19.2: 140-146.
[5]Cortes, Jesus, et al. An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea [J]. 1990: 176-178.
[6]Khosla, Chaitan, Shiven Kapur, and David E. Cane. Revisiting the modularity of modular polyketide synthases [J]. Current opinion in chemical biology, 2009, 13.2: 135-143.
[7]Menzella, Hugo G., et al. Redesign, synthesis and functional expression of the 6-deoxyerythronolide B polyketide synthase gene cluster [J]. Journal of Industrial Microbiology and Biotechnology,2006, 33.1: 22-28.
[8]Oliynyk, Markiyan, et al. A hybrid modular polyketide synthase obtained by domain swapping [J]. Chemistry & biology,1996, 3.10: 833-839.
[9]Lau, Janice, David E. Cane, and Chaitan Khosla. Substrate specificity of the loading didomain of the erythromycin polyketide synthase [J]. Biochemistry, 2000, 39.34: 10514-10520.
[10]Nowak-Thompson, Brian, et al. Characterization of the pyoluteorin biosynthetic gene cluster of Pseudomonas fluorescens Pf-5 [J]. Journal of bacteriology, 1999, 181.7: 2166-2174.
[11]Caffrey, Patrick, et al. Amphotericin biosynthesis in Streptomyces nodosus deductions from analysis of polyketide synthase and late genes [J]. Chemistry & biology,2001, 8.7: 713-723. [12]Dunn, Briana J., et al. Comparative analysis of the substrate specificity of trans-versus cis-acyltransferases of assembly line polyketide synthases [J]. Biochemistry,2014.
[13]Jiang, Ming, and Blaine A. Pfeifer. Metabolic and pathway engineering to influence native and altered erythromycin production through E. Coli [J]. Metabolic engineering, 2013, 19: 42-49. [14]Chen, Xianzhong, et al. Metabolic engineering of Escherichia coli: A sustainable industrial platform for bio-based chemical production [J]. Biotechnology advances, 2013, 31.8: 1200-1223.
